Establishment of foreign-gene-free genome editing technology in Japanese gentian

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39030:Horticultural science-related
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: Takahashi Shigekazu 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 研究員 (10600033)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: CRISPR/Cas9 / Gentian / Genome editing

Outline of Final Research Achievements

CRISPR / Cas9, one of the genome editing technologies, is widely used in the world because it is easy to operate. Generally, genome-edited plants carry foreign genes, however it is required genome edited plants with no foreign gene for practical use. Furthermore, in vegetative propagation plants and horticultural crops with high heterosis, it is important to obtain foreign gene-free genome edited lines in the present generation. Therefore, we performed the Agrobacterium method using Japanese gentian and tobacco as materials under various conditions, and tried to obtain foreign gene-free genome edited lines in the present generation. As a result, several signals for genome editing were detected in the shoots in the early stage of redifferentiation, however these signals disappeared with growth, and it was not possible to obtain genome edited plants with no foreign gene in the present generation.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究により当代での外来遺伝子フリーのゲノム編集体の取得において問題となる複数の課題をあぶり出すことができた。本知見をフィードバックすることで、今後の当該分野の研究の進展が期待される。