Elucidating the molecular mechanisms of host plastid collapse and starch degradation by phytopathogenic filamentous fungi

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Ehime University
• Principal Investigator: Yaeno Takashi 愛媛大学, 農学研究科, 准教授 (10404063)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: オオムギうどんこ病菌 / デンプン / プラスチド崩壊 / アミラーゼ / エフェクター

Outline of Final Research Achievements

To elucidate the molecular mechanisms underlying the reduction of starch in the plastids in epidermal cells during the penetration of barley powdery mildew pathogen (Bgh), transgenic barley lines expressing plastid-localized GFP and starch-bound mCherry were observed after infection with Bgh. Dispersed GFP fluorescence was observed near the site of penetration, but mCherry fluorescence smaller than the plastid was observed dispersing into the cell, suggesting that starch was leaked out of the collapsed plastid. For the analysis of APEC29 activity in vitro, recombinant protein expressing using E. coli has become an inclusion body, so we continue to try protein synthesis using other expression system.

Free Research Field

植物病理学

Academic Significance and Societal Importance of the Research Achievements

本研究では、オオムギうどんこ病菌の侵入時に宿主表皮細胞内のプラスチドが崩壊してデンプンが露出することが明らかになった。この研究によって栄養摂取メカニズムの全容が解明されることにより、表皮細胞に特化した防除法の開発につなげることができる。宿主表皮細胞内にあるデンプンの分解、吸収を阻害するような技術開発に貢献できると考えられる。