Functional analysis of a new AAA-ATPase gene in rice defense response

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: JIANG Chang-Jie 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 主席研究員 (80370659)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: OsAAA-ATPase / サリチル酸 / イネ / いもち病 / 防御応答

Outline of Final Research Achievements

A novel AAA-ATPase family gene OsAAA-ATPase1 was identified which responded to rice blast fungus infection in a salicylic acid (SA)-dependent manner. The expression of OsAAA-ATPase1 showed an SA-specific response under the control of OsNPR1 and WRKY45. OsAAA-ATPase1 protein was shown to have ATPase activity and be localized in the cytoplasm. Overexpression of this gene in rice plants markedly enhanced resistance to blast and bacterial leaf blight, while suppression of its expression reduced the resistance. Furthermore, transcriptome analysis showed that OsAAA-ATPase1 induces disease resistance by activating various signaling and metabolic pathways including ethylene signaling pathways.

Free Research Field

植物保護科学

Academic Significance and Societal Importance of the Research Achievements

本研究で初めてサリチル酸シグナル伝達経路におけるAAA-ATPase ファミリー遺伝子（OsAAA-ATPase1）の機能を明らかにされた。これらの成果は植物防御応答におけるサリチル酸シグナル伝達機構の全容を解明する上で重要な新知見をもたらすものである。また、新規に見出されたOsAAA-ATPase1遺伝子はイネ病害抵抗性を正に制御することが明らかになり、耐病性分子育種への活用が期待される。