2023 Fiscal Year Final Research Report
Development of a red tide-predicting method based on potential for resting cyst germination of a psychrotrophic red tide-causing microalga
| Project/Area Number |
19K06211
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 40030:Aquatic bioproduction science-related
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2024-03-31
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| Keywords | 好低温性赤潮 / 生活環 / 休眠期 / 定量PCR / トランスクリプトーム / 遺伝子発現 / 赤潮予測 |
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| Outline of Final Research Achievements |
A psychrotrophic dictyochophyte Pseudochattonella verruculosa is known as a causative agent of red tides in high latitude or winter marine waters. In this study, a method was developed to specifically quantify the microalga with a quantitative PCR (qPCR) targeting the plastid genome DNA after determining its partial nucleotide sequences. Then, comprehensive transcriptome analyses as well as photosynthesis-related gene-targeting qPCR were done to clarify gene expression profiles at different stages in the microalgal cell cycle. The results showed that basal metabolic activities were reduced at a possibly resting, cell aggregate-forming stage, while expression of molecular chaperon- and photosynthesis-related genes were induced. These findings will contribute to developing a method to predict red tides at an initial stage of their outbreaks as well as to prevent microalgal blooming by controlling production and release of unicellular, swimming vegetative cells from the cell aggregates.
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| Free Research Field |
水圏環境微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
P. verruculosaは、サケ養殖などが盛んな海外の養殖漁場で甚大な赤潮被害をもたらしているほか、近年我が国でもブルームの形成、それに伴う漁業被害が報告されるようになった。しかしながら、その生理生態や生活環など不明な点が多い。本研究では、遺伝子レベルでの本藻特異的な検出・定量化法を確立し、生活環各段階に特有の遺伝子発現パターンを明らかにした。これらの知見は、細胞レベル、遺伝子レベルでの生活環や生理生態の解明に繋がる。さらには、赤潮予測技術の開発やシード・ポピュレーションとして機能する休眠期細胞の制御による赤潮防除法の確立にも資すると期待される。
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