Molecular characterization of the soybean gene of unknown function involved in response and tolerance to salt stress

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06330
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 41050:Environmental agriculture-related
• Research Institution: Ibaraki University
• Principal Investigator: Kojima Toshio 茨城大学, 農学部, 准教授 (70311587)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: ダイズ / 塩ストレス / 耐塩性 / 機能未知遺伝子 / アルマジロリピート / ユビキチン－プロテアソーム

Outline of Final Research Achievements

In this study, we investigated the molecular response and tolerance mechanism of the GmTDF-5 gene, a soybean gene of unknown function, under salt stress. Spatial and temporal expression of GmTDF-5 gene in transgenic Arabidopsis under salt stress showed mainly in aerial organs, especially in the xylem in vascular bundles. Overexpression of GmTDF-5 conferred altered transcription level of genes involved in pectin metabolism for mechanical properties of the cell wall and in regulators for phytohormones.

Free Research Field

環境農学

Academic Significance and Societal Importance of the Research Achievements

本成果は、機能未知遺伝子GmTDF-5による塩ストレス応答・耐性機構の活性化プロセスを理解する上で重要な知見になると考える。形質転換シロイヌナズナで見られたGmTDF-5の機能発現による耐塩性上昇のプロセスを適切にコントロールできれば、ダイズの潜在能力を引き出した新たな耐塩性品種をデザインできるかもしれない。