Studies of chitin degradation mechanism of Paenibacillus sp.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K06340
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 41050:Environmental agriculture-related
• Research Institution: Fukui Prefectural University
• Principal Investigator: Itoh Takafumi 福井県立大学, 生物資源学部, 教授 (10402827)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: alginate / alginate lyase / chitin / chitin binding protein / glucanase / Paenibacillus

Outline of Final Research Achievements

The following results were obtained in this study.(1) Analysis of chitin oligosaccharide degrading enzyme: P. FPU-7 degrades chitin oligosaccharides to monosaccharides by GlcNAcase NagZ in the cytosol.(2) Analysis of alginate lyase: The alginate lyase of P. FPU-7 was characterized and the enzymatic products of alginate promoted the elongation of rice and spinach roots.(3) Analysis of chitin oligosaccharide-binding proteins: The chitin oligosaccharides recognition mechanisms of two chitin oligosaccharide-binding proteins (NagB1 and NagB2) were determined.(4) Analysis of two-component regulatory protein: Analysis of the interaction between the two-component regulatory protein NagS and chitin oligosaccharides suggested that NagS recognizes to chitin oligosaccharides through NagB1.

Free Research Field

環境農学

Academic Significance and Societal Importance of the Research Achievements

土壌細菌Paeibacillus sp. str. FPU-7は、カニ殻由来の固体状のキチンをも強力に分解する。P. FPU-7のキチン分解を制御し、有用なキチンオリゴ糖を効率的に生産するためには、分解されたキチンがどのように取り込まれ、どのように利用されているのかも明らかにする必要がある。今回、P. FPU-7が細胞表層でどのようにキチンを認識しているのかについて、その一端を明らかにした。また、取り込んだオリゴ糖を分解する酵素やアルギン酸分解酵素についてもその諸性質と立体構造を決定した。これらの成果は、より効率的で環境負荷の少ない酵素による多糖分解法の確立に繋がることが期待される。