Identification of causal mutation for gene expression change associated with bovine marbling

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K06351
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Niigata University
• Principal Investigator: Yamada Takahisa 新潟大学, 自然科学系, 教授 (40253207)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: 脂肪交雑 / 遺伝子発現 / 原因変異

Outline of Final Research Achievements

This study showed that EDG1 and TTN polymorphisms, possible candidates for causal mutations of bovine marbling, respectively, are associated with mRNA level and protein level of EDG1 gene and those of TTN gene. On the other hand, this study demonstrated no effect of these polymorphisms (EDG1 and TTN polymorphisms) on EDG1 and TTN gene transcription. Thus, we suggested that these polymorphisms (EDG1 and TTN polymorphisms) are not regarded as causal mutations of gene expression change of EDG1 and TTN genes, and then of bovine marbling. DNA polymorphisms, which are in linkage disequilibrium with EDG1 and TTN polymorphisms, may be true polymorphisms for causal mutations of bovine marbling.

Free Research Field

動物遺伝学

Academic Significance and Societal Importance of the Research Achievements

EDG1の発現増加、TTNの発現減少は、それぞれ、筋肉内血管形成、筋線維損傷を引き起こすことにより、脂肪交雑形成を誘導すると示唆されている。EDG1多型、TTN多型はそれぞれの遺伝子の発現変動さらには脂肪交雑形成能力と相関がみられ、EDG1多型、TTN多型と連鎖不平衡にある近傍のDNA多型が、真の発現変動および脂肪交雑形成能力の原因変異になると考えられるが、EDG1多型、TTN多型は、脂肪交雑形成能力の向上を目指したDNA育種技術のための分子マーカーとして有用であると考える。