2021 Fiscal Year Final Research Report
Analysis and application of follicular somatic cell-dependent oocyte metabolic regulation and epigenome regulation controled by iron during follicular development.
| Project/Area Number |
19K06359
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42010:Animal production science-related
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| Research Institution | Prefectural University of Hiroshima |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
島田 昌之 広島大学, 統合生命科学研究科(生), 教授 (20314742)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 卵胞発育 / 排卵 / 卵子成熟 / トランスフェリン / エピジェネティック制御 |
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| Outline of Final Research Achievements |
IVM of COCs is an important for embryo production of domestic animals. In pig, since blastocyst rate following IVF is still low, there is a need to improve the developmental potential of oocyte produced from IVM. Since we previously found the accumulation of transferrin (TF) in developing follicle in pig, it assumed that the TF-Fe3+ is important for follicular development and/or ovulation. To investigate this, we firstly examined the basic role of Tf-Fe3+ on follicular development and ovulation using null mice of granulosa and cumulus cell specific-TF receptor knockout (Tfr1cKO) mice. Tfr1cKO mice failed to follicular development caused by the downregulation of Fshr and Cyp19a1 in granulosa cells. To utilize the knowledge obtained from basic research using Tfr1cKO mice for the development of IVM technique of domestic aminals, we developed a two-step culture method as novel IVM combines preIVM and subsequent IVM supplemented with TF-Fe3+, which can efficiently produce blastocyst.
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| Free Research Field |
動物生産科学，生殖生理学，生殖内分泌学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，TFにより供給される鉄は卵胞発育に重要であることを初めて明らかにした。加えて，TFにより供給される鉄は卵胞発育を誘導するために重要なFSHRのエピジェネティック制御に関わる可能性が示されたことから，哺乳動物の卵胞発育を誘導する根幹に関わり学術的意義は高い。また，これまでブタCOCを培養した際の胚盤胞率は20%から高くても30%程度であったが，TFを考慮したpreIVMとIVMを組み合わせた2ステップ培養により49%までその率を向上させたことから，ブタのみならずウシの効率的生産やヒトの高度生殖補助医療にも応用可能であると考えられ，社会的意義の高い成果であると言える。
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