2021 Fiscal Year Final Research Report
Understanding of the pathogenic mechanism of canine mammary cancer using organoid culture and construction of the foundation for cancer stem cell-target therapy
| Project/Area Number |
19K06391
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42020:Veterinary medical science-related
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 乳腺腫瘍 / 犬 / オルガノイド / 癌幹細胞 |
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| Outline of Final Research Achievements |
Mammary tumors occur frequently in female dogs, where such tumors exhibit complexity when examined histologically. These tumors are composed not only of proliferative luminal epithelial cells, but also of myoepithelial cells and/or mesenchymal cells with cartilage and osseous tissues. The origin of this complexed histogenesis remains speculative. Understanding the properties of organoid containing cancer stem cells is of paramount importance.
In the three-dimensional organoid cultures derived from mammary tumors, the organoids showed spherical or irregular shapes, and histologically luminal formation and solid proliferation. These cells consisting of the organoids were immunohistochemically positive for CK19 and p63, suggesting the presence of both luminal epithelial and myoepithelial cells. Therefore, organoid formed in this study mimicked the tumor tissues of mammary tumor resected surgically.
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| Free Research Field |
獣医腫瘍学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、犬に特有な乳腺腫瘍の発症・病態形成機構の解明に加え、乳癌幹細胞を標的とした分子標的薬を抽出し、獣医療における新規癌治療戦略の基盤構築に貢献できる。さらに、ヒトの癌基礎研究だけでなく、ヒト医療に社会還元することが期待できる極めて重要な獣医学から医学へのトランスレーショナル研究である。
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