2021 Fiscal Year Final Research Report
Mechanism of liver function improvement by cooperation of bovine hepatic sinusoidal cell lines
Project/Area Number |
19K06431
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 42020:Veterinary medical science-related
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
Yoshioka Miyako 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, 室長 (80355198)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 共培養 / 肝類洞細胞株 / 急性期蛋白質 / 炎症性サイトカイン |
Outline of Final Research Achievements |
We found that the combination of BH 4 cells (a parenchymal cell line) and B 46 cells (a non-parenchymal cell line) was optimal for enhancing mRNA expression of acute-phase proteins, Serum amyloid A and Haptoglobin, in coculture of bovine liver-derived cells. It was revealed that the enhancing effect involves contact between cells and involvement of the inflammatory cytokines IL -6 and IL -1 β secreted by B 46 cells. Metabolomic analysis of intracellular metabolites showed that multiple metabolites were increased by LPS stimulation in coculture compared with monoculture, suggesting that ATP production and nucleic acid synthesis were enhanced.
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Free Research Field |
獣医学
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Academic Significance and Societal Importance of the Research Achievements |
ウシ肝臓由来の肝実質細胞株と非実質細胞株の共培養において、肝機能の向上が期待できる組み合わせが明らかとなった。この共培養系を用いることで、ウシ特有の代謝機能を保持した簡便なin vitroアッセイが可能となり、ウシの肝機能を保全する飼養方法や有害物質による肝障害の予防・治療法の開発につながる可能性がある。
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