Robust self-renewal in 129 strain derived ESCs established by Esrrb

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06459
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42040:Laboratory animal science-related
• Research Institution: Kyoto Prefectural University of Medicine (2020-2021); Kanazawa Medical University (2019)
• Principal Investigator: OHTSUKA SATOSHI 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (40360515)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: ES細胞 / LIFシグナル

Outline of Final Research Achievements

Mouse embryonic stem cells (mESCs) have been established in conventional serum culture. mESCs derived from 129 mouse strain are stably self-renewing in serum culture. In contrast, mESCs from non-obese diabetic (NOD) mouse strain is not yet maintained in serum culture. In this project, we tried to explore why ESCs from 129 strain were stably established and continued to self-renew in serum culture. We identified transcription factor Esrrb which was specifically retained in 129-ESCs. Maintaining the expression of Esrrb in 2i-established NOD-ESCs allowed self-renewal in an inhibitor-free serum condition. Esrrb enhanced LIF-Stat3 activity in NOD-ESCs at a similar level to that in 129-ESCs. These results indicate that Esrrb supports 129-ESCs self-renewal in serum through enhancing LIF-Stat3 as well as Tfcp2l1 does.

Free Research Field

幹細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた成果をもとに、ナイーブ型幹細胞の樹立と維持が、ヒトを含む哺乳類からも可能となり、再生医学へ大きく貢献できると考えている。