2021 Fiscal Year Final Research Report
Identification of structures of advanced glycation end-products responsible for proatherosclerotic effects - analysis using aptamers.
| Project/Area Number |
19K06461
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42040:Laboratory animal science-related
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
東元 祐一郎 久留米大学, 医学部, 教授 (40352124)
外川内 亜美 久留米大学, 医学部, 助教 (60809177)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 動脈硬化 / 終末糖化産物 / 核酸医薬品 |
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| Outline of Final Research Achievements |
We aimed to identify the structures responsible for the action of glyceraldehyde-derived glycation end products (GLA-AGEs), which are closely related to the onset and progression of atherosclerosis. For this purpose, the effects of three known GLA-AGEs structures were elucidated using cultured human cells. In addition, aptamers (single-stranded DNA) that bind to GLA-AGEs structures were created, and their inhibitory effects on the actions of GLA-AGEs structures and a novel method for quantifying GLA-AGEs structures, which had been difficult to quantify in vivo, were developed.
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| Free Research Field |
血管生物学
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| Academic Significance and Societal Importance of the Research Achievements |
動脈硬化症は心血管病の基盤となる疾患であり、本邦において主たる死因の一つである。しかし、その発症メカニズムは様々な生活習慣病と関わっており、解明と治療法の開発は困難である。そのため、多くの生活習慣病と動脈硬化症をつなぐ共通の病的因子を同定し、それに対する特異的な治療法の開発が望まれてきた。今回我々は、生体内の老化物質である3つの終末糖化産物に着目し、それぞれに結合する新規の核酸医薬品であるアプタマーを作成し、阻害効果と、アプタマーを応用した終末糖化産物の定量方法の開発をおこなった。動脈硬化症と生活習慣病をつなぐ共通因子の解明と、新規の動脈硬化症の治療方法を提供に繋がる成果を得られた。
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