2021 Fiscal Year Final Research Report
Phenotypic analysis of newly generated c-kit mutant marmosets
Project/Area Number |
19K06462
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 42040:Laboratory animal science-related
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Research Institution | Central Institute for Experimental Animals |
Principal Investigator |
Kumita Wakako 公益財団法人実験動物中央研究所, マーモセット医学生物学研究部, 研究員 (50549128)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | コモンマーモセット / c-kit遺伝子 / ゲノム編集 |
Outline of Final Research Achievements |
Phenotypic analyses of c-kit gene-modified common marmosets (c-kit marmosets), generated by gene-editing technology, were performed. Hematopoietic function analysis showed no difference between c-kit and wild-type marmosets. To clarify the phosphorylation ability of mutant c-kit exhibited by the c-kit marmoset, we performed a phosphorylation assay using a mutant c-kit expression vector and a phosphorylated c-kit antibody. The results showed that one mutant c-kit shown by males and all mutant c-kit proteins shown by females lacked phosphorylation ability. For reproductive function analysis, semen was obtained from a male c-kit marmoset and analyzed, and the mutant c-kit gene was detected. In female c-kit marmosets, urinary progesterone levels were measured, suggesting possible infertility. This study indicates that the c-kit marmoset may be a helpful model in reproductive research. It is expected to contribute to establishing c-kit mutant primate models in the future.
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Free Research Field |
実験動物
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Academic Significance and Societal Importance of the Research Achievements |
c-kitマーモセットをもちいた本研究の結果から、c-kit変異マウスとは違う表現型が確認された。これは、霊長類ならではの表現型を見出した可能性が考えられ、ヒトへの応用が期待できる。また不妊のc-kitマーモセットは人工樹立した生殖細胞や、幼若生殖細胞の移植レシピエントに使用できると考えられ、不妊症の原因究明や治療法開発の研究や、霊長類実験動物の問題点である世代時間を短縮する研究などに貢献することが期待できる。
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