2021 Fiscal Year Final Research Report
Structural insights into the transcription-translation complex revealed by cryo-EM
| Project/Area Number |
19K06518
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43020:Structural biochemistry-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | クライオ電子顕微鏡 / 翻訳 / 転写 / リボソーム / RNAポリメラーゼ / RNA |
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| Outline of Final Research Achievements |
In central dogma, transcription that copies genetic information encoded on DNA to RNA having the corresponding sequence, and translation that subsequently converts the nucleic acid sequence into the amino acids sequence that forms proteins, those are important processes playing fundamental roles in gene expression. In prokaryotes, both of these processes take place in the cytoplasm, so the machineries involved in transcription and translation are spatially close to each other and work in a coordinated manner. In this research project, the structure-function relationship between RNA polymerase, which controls transcription, and ribosome, which controls translation, was investigated by using single particle cryo-EM analyses. mRNA exposed from RNA polymerase was visualized at high resolution as it was incorporated into ribosomes.
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| Free Research Field |
構造生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は生命の根幹を担うセントラルドグマにおいて、超分子複合体がどのように機能するのかを解明するため、生物学的に非常に重要な課題に取り組んでいると言える。また原核生物特有のシステムであることから、ヒトの生命システムに影響を与えない副作用のない抗菌化合物の開発に有用な情報を取得出来るため、創薬に資する社会的意義のある研究であると言える。
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