Analysis of hypoxia tolerance mechanism based on sulfur-dependent energy metabolism

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06537
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43030:Functional biochemistry-related
• Research Institution: Osaka Prefecture University
• Principal Investigator: Kasamatsu Shingo 大阪府立大学, 理学(系)研究科(研究院), 助教 (80738807)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 活性硫黄分子 / 超硫黄分子 / 低酸素

Outline of Final Research Achievements

We developed a novel tyrosine-based alkylating agent, N-iodoacetyl tyrosine methyl ester (TME-IAM) by a coupling reaction of TME and iodoacetic acid. Mass spectrometric analyses revealed that TME-IAM can derivatize reactive sulfur species without artificial decomposition in biological samples. By quantitative analysis for reactive sulfur species, the results showed that there were differences in the profile of reactive sulfur species between hypoxia-tolerant golden hamster kidney cells and hypoxia-vulnerable human kidney cells. These results suggest that reactive sulfur speceies may be involved in the hypoxia torelance mechanism in hibernators.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究課題で開発したTME-IAMを用いることで、これまで検出が困難であった生体試料中の活性イオウ分子種を正確に検出することを可能となる。活性イオウ分子種は強力な抗酸化活性やレドックスシグナル制御活性を示すことが知られていることから、本検出試薬を用いた活性イオウ分子種メタボーム解析系は、酸化ストレス関連疾患や、がんなどの疾病・病態における活性イオウ分子種の生物学的意義を解析する上で非常に有用であると期待される。