Development of a comprehensive functional analysis method for rice membrane transporters using the yeast protein expression library.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K06565
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43030:Functional biochemistry-related
• Research Institution: Shimane University
• Principal Investigator: Takashi Akihiro 島根大学, 学術研究院農生命科学系, 助教 (40508941)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 膜輸送体 / チャンネル / トランスポーター

Outline of Final Research Achievements

The yeast protein expression library that expresses about 1500 rice membrane transporters was developed and used this librarye to screen for transporters of insecticides. This library enables negative screening, which has not been possible in previous studies. It is possible to efficiently screen yeast for susceptibility on selection media in a short period of time. In this study, we selected yeasts susceptible to insecticides and generated rice plants lacking or overexpressing the transporter, and found that the amount of insecticide absorption was reduced when the transporter was deficient and increased when it was overexpressed. The constructed library proved to be an effective screening method.

Free Research Field

植物分子生物学

Academic Significance and Societal Importance of the Research Achievements

イネには約1500種類の膜輸送体があると考えられている。そのうち、機能が明らかになっている輸送体は30%程度であり、機能が不明な膜輸送体が数多く存在する。膜輸送体の欠損変異体を用いた研究は膜輸送体研究を行ううえで最も強力な研究材料となるが、１つの膜輸送体を欠損しても表現型が現れない場合もある。本研究で構築した実験手法は逆遺伝学的な手法を用いた手法であり、本ライブラリーを構築できたことで、これまで機能が明らかになっていなかった膜輸送体の機能を次々に明らかにできるものと考えられる。