Regulation of Raf-1 kinase through PP1 signaling

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K06573
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43030:Functional biochemistry-related
• Research Institution: Okayama University of Science
• Principal Investigator: Eto Masumi 岡山理科大学, 獣医学部, 教授 (20232960)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: Cell signaling

Outline of Final Research Achievements

The goal of this study was to understand the function of PHI-1, a regulatory protein of PP1, the major Ser/Thr phosphatase, and its role in the cell. PHI-1 has been suggested to play important roles in cellular processes and signal transduction, although the function was largely unknown. In this study, we performed experiments including expression analysis of PHI-1, gene knockdown, association with the EGF signaling pathway, and analysis of Raf-1 regulation by PHI-1, providing new insights into the function of PHI-1 and its intracellular role. In addition, we developed a new unknown kinase detection method.

Free Research Field

Molecular physiology

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、PP1調節タンパク質であるPHI-1の機能解明に貢献しました。PHI-1の細胞内での役割が明らかになることで、PP1を介した細胞のシグナル伝達や病理学的な変化に関与するメカニズムを理解する上で重要な情報となります。さらに、PHI-1が関与する細胞増殖やアポトーシス制御などの生理的プロセスの理解は、がんや他の疾患の発症や進行に関連することが示唆されました。したがって、本研究の成果は、細胞生物学や病態生理学の研究分野において新たな知識を提供し、将来的には新しい治療法や治療標的の開発につながる可能性があります。