2021 Fiscal Year Final Research Report
Development of ATP super-resolution imaging method using atomic force microscopy and chemiluminescent proteins
| Project/Area Number |
19K06580
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43040:Biophysics-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 原子間力顕微鏡 / 超解像顕微鏡 |
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| Outline of Final Research Achievements |
I have worked on developing microscopy capable of measuring the nanoscale distribution of adenosine triphosphate (ATP) using an atomic force microscope (AFM) and a luciferase protein. By using a focused ion beam system, I succeeded in developing a technique to bind the biosensor proteins only to a region of about 100 nm at the tip of the AFM cantilever. In addition, I incorporated a home-made AFM into a commercially available confocal microscope and constructed a confocal-AFM simultaneous measurement system for cells. I also developed a device to emit ATP-containing solution from a hole of less than 100 nm in diameter, which enabled us to verify the theory of this microscopy. On the other hand, it became clear that the biosensor initially planned to be used was not suitable for this technology.
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| Free Research Field |
生物物理学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で開発したAFM探針先端へのタンパク質の局所的な吸着方法はrecognition imagingを始めとするAFM探針を用いたイメージング技術にとって基盤的技術になる可能性を持つ。現時点では化学的修飾方法を用いたセンサー付加が行われているが、本研究で開発した方法を用いることによって比較的簡便にしかも数十ナノメートルの精度で目的タンパク質を結合させることが可能になる。さらに本研究で構築した共焦点-AFM融合顕微鏡は近年増加しつつある細胞のAFM観察を行う上で基本的な機器となると考えられる。
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