2022 Fiscal Year Final Research Report
Endogenous degradation signals in proteasome substrate
Project/Area Number |
19K06595
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 43040:Biophysics-related
|
Research Institution | University of Toyama |
Principal Investigator |
Inobe Tomonao 富山大学, 学術研究部工学系, 准教授 (50568855)
|
Project Period (FY) |
2019-04-01 – 2023-03-31
|
Keywords | プロテアソーム / 細胞内タンパク質分解 / Unstructured領域 / ユビキチン-プロテアソーム系 / ユビキチン / タンパク質工学 |
Outline of Final Research Achievements |
The ubiquitin-proteasome system (UPS) is the most important proteolytic system in eukaryotes, and it has been believed that the selection of target proteins for the UPS-mediated degradation is performed solely by the ubiquitination system and that ubiquitinated proteins are recognized and degraded by the proteasome. However, a number of reports have suggested that the properties of the substrate proteins themselves are also important for substrate recognition by the proteasome. In this study, we found that the charge of the substrate protein and the amino acid sequence of the unstructured region have a significant effect on its degradation by the proteasome.
|
Free Research Field |
タンパク質科学
|
Academic Significance and Societal Importance of the Research Achievements |
プロテアソームの分解ターゲット蛋白質のUnstructured領域や電荷が分解に大きな影響を与えることを示した本研究の成果は、プロテアソームによる分解は今まで考えられていた以上に高度かつ多段階に制御されていることを示唆しており、プロテアソームによる分解と関与する様々な生命現象の理解に一石を投じるだろう。さらにはUnstructured領域や電荷に注目した分解制御方法の開発が可能になり、基礎研究のみならず、人の疾病とその治療法への応用が期待される。
|