2021 Fiscal Year Final Research Report
Stability and flexibility of the DNA replication program revealed by single-cell DNA replication sequencing in vivo
| Project/Area Number |
19K06610
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43050:Genome biology-related
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| Research Institution | Mie University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
平谷 伊智朗 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (40583753)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | DNA複製 / 染色体 / シングルセル |
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| Outline of Final Research Achievements |
We investigated structural characteristics of mammalian chromosomes at the level of individual cells using a novel technique for analyzing replication timing domains (chromosomal unit defined by DNA replication timing). To our surprise, cell-type-specific replication timing domain organization was remarkably conserved between individual cells and even between homologous chromosomes. A small degree of cell-to-cell variation was also detected in specific genomic regions where replication timing shifts during cell differentiation. Thus, our study uncovered the previously unrecognized dynamic and static properties of chromosomal domains.
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| Free Research Field |
分子細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
これまでDNA複製制御を単一細胞レベルで、高解像度、広範囲にわたって詳細に解析した例はなく、本研究で得られた成果は今後のDNA複製研究に大きく貢献することは確実である。DNA複製に着目することで、これまでアプローチの難しかった個々の細胞レベルでの染色体構造の安定性や揺らぎの実体を明らかにしたことは、巨大な染色体分子の動態を理解するためのモデリングやシミュレーションにとって非常に有用で、関連学問分野への波及効果が期待できる。
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