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2021 Fiscal Year Final Research Report

Elucidation of complex gene expression regulation dynamics through higher-order genome structure

Research Project

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Project/Area Number 19K06612
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43050:Genome biology-related
Research InstitutionHiroshima University

Principal Investigator

Ochiai Hiroshi  広島大学, 統合生命科学研究科(理), 准教授 (60640753)

Project Period (FY) 2019-04-01 – 2022-03-31
Keywordsゲノム構造 / 遺伝子発現 / 多能性幹細胞 / 転写 / ライブイメージング
Outline of Final Research Achievements

In this study, we aimed to elucidate the mechanism of transcriptional regulation by Nanog genes and their interacting regions in mouse ES cells by using sequential-DNA-RNA-FISH and a STREAMING-tag system for visualizing the nuclear localization and transcriptional activity of specific endogenous genes. We developed the STREAMING-tag system and found that RPB1 and BRD4 cluster in the vicinity of Nanog genes only when the gene is in a transcriptionally active state, and published a paper on a preprint server (Ohishi et al., bioRxiv, 2022).

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では、独自に開発した遺伝子イメージング技術を利用して、遺伝子活性状態依存的に特定因子が遺伝子近傍でクラスターを形成することを見出した。本成果は遺伝子発現動態がどのように制御されているかを解く鍵となりえる成果である。遺伝子発現制御機構はあらゆる生命現象に関わる基本的なプロセスであり、大きな学術的意義があるといえる。

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Published: 2023-01-30  

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