2021 Fiscal Year Final Research Report
The regulatory origin of cephalopod limbs
| Project/Area Number |
19K06620
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43050:Genome biology-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
MARLETAZ FERDINAND 沖縄科学技術大学院大学, 分子遺伝学ユニット, 客員研究員 (90833955)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | Cephalopod / limb / evolution / regulation |
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| Outline of Final Research Achievements |
Our plan was to obtain RNA-seq and ATAC-seq library for each limb bud of a bobtail squid embryo in order to predict differences in transcription factor binding that would be involved in arm identity. We wanted to understand how a novel patterning could be deployed . We performed these experiments and sequenced corresponding libraries. We performed peak calling and differential peak activation analysis, which has been coupled with foot printing analysis. We identified some key genes and ordered corresponding probes for HCR, an advanced in situ hybridisation technique. In parellel, we optimised protocols for HCR imagining of squid embryos and fixed a lot of embryos. Some key results have been integrated in a paper about squid single-cell sequencing that will be submit in 2022.
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| Free Research Field |
Genomics, evodevo
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| Academic Significance and Societal Importance of the Research Achievements |
Development of new cephalopod model and new molecular biology techniques. Cephalopod have a strong cultural important (especially in Japan). Better understanding of biological novelties at regulatory level
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