2021 Fiscal Year Final Research Report
Development of a novel technology to capture RNA-chromatin interactions
Project/Area Number |
19K06623
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 43050:Genome biology-related
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Kato Masaki 国立研究開発法人理化学研究所, 生命医科学研究センター, 上級研究員 (10625437)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | ノンコーディングRNA / 新生鎖RNA / クロマチン |
Outline of Final Research Achievements |
With the help of sequencing technologies, thousands of lncRNAs have been identified. Most of them are present in the nucleus, and up to 40% of lncRNAs exert their functions by binding to chromatin. The interactions of lncRNAs with their target chromatin DNA are not clear. Therefore, the function of most lncRNAs remains unknown. Here, we developed a novel method to capture RNA-chromatin interactions at a genome-wide scale called RADICL-seq (RNA And DNA Interacting Complexes Ligated and sequenced). RADICL-seq is a proximity ligation-based methodology that broadly identifies the target genomic regions for coding and ncRNAs. We made RADICL libraries of several cell lines from mouse, human and Drosophila. We demonstrated the compatibility of this technology to map ncRNAs roX in Drosophila S2 cells and Xist lncRNA in mouse female cells.
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Free Research Field |
ゲノム生物学
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Academic Significance and Societal Importance of the Research Achievements |
本研究ではRNAとクロマチンの相互作用を網羅的に検出する系RADICL-seq法を構築した。これまで核内RNAとクロマチンの相互作用の検出方法としてはChIRP-seqやCHART-seq法など1つのRNAをターゲットにした解析法しか存在しなかったがRADICL-seq法の開発により多くのRNAを同時に網羅的に解析することが可能となった。また免疫沈降法と組み合わせて新しい技術に応用することで、よりターゲットを絞った解析が可能となった。
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