Analysis of the mechanism that determines cilia localization of G protein-coupled receptors

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06636
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44010:Cell biology-related
• Research Institution: Osaka University
• Principal Investigator: MIYOSHI KO 大阪大学, 連合小児発達学研究科, 助教 (90362996)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: Ｇ蛋白質共役型受容体

Outline of Final Research Achievements

In order to elucidate the mechanism by which specific G protein-coupled receptors localize to primary cilia, molecules that upregulate ciliary localization of serotonin receptor type 6 (Htr6) were explored. Yeast two-hybrid screening identified γ-adaptin as an interacting molecule of a region in the fourth intracellular domain of Htr6 that is important for ciliary localization of Htr6. By analysis of localization in hTERT-RPE1 cells knocked-out for γ-adaptin, it was suggested that localization of Htr6 to primary cilia is upregulated through the action of γ-adaptin on the fourth intracellular domain of Htr6.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

Ｇ蛋白質共役型受容体の繊毛への「搬入」や「搬出」に必要な蛋白質として、Tubby-like protein 3や繊毛病Bardet-Biedl症候群の原因遺伝子産物が報告されている。一方、本研究では、未だ不明な点が多い、特定のＧ蛋白質共役型受容体が１次繊毛に局在する機構に焦点を当てた。
本研究で得られた成果は、小胞輸送において、積荷であるＧ蛋白質共役型受容体に対するアダプター蛋白質複合体の作用が、特定の受容体の１次繊毛への局在に関与する可能性を示唆するものである。