2021 Fiscal Year Final Research Report
Establishment of medaka lifespan measurement system
| Project/Area Number |
19K06658
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | メダカ / 老化 / 寿命 |
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| Outline of Final Research Achievements |
This project aims to enable the analysis of aging and longevity using medaka fish, a vertebrate with a relatively short life span.
First, we identified a medaka homolog of the p16 gene, a marker of senescent cells. Then, we created transgenic fish harboring a Venus fluorescent protein gene under the p16 promoter. Finally, to determine the effect of caloric intake on the lifespan of medaka, we kept medaka with various amounts of food.
We confirmed that medaka's lifespan was extended when they were fed less food than when they had more food.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
老化や寿命のメカニズムを短期間で解析するためには短期間に自然老化する生き物を用いることが望ましい。本研究ではメダカを用いることで寿命・老化解析が行えるかを検証した。
まず、メダカにおける老化細胞マーカー遺伝子ホモログのクローニングに成功したため、これを用いた老化細胞生成メカニズムの解析が可能になった。また、餌の量を変化させることで寿命が変化することが観察できた。これらによりメダカを用いた寿命・老化解析を行うための手法が一部確立された。
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