2023 Fiscal Year Final Research Report
Identification of partner molecules that bind directly to GRHL3 factors during epidermal differentiation.
| Project/Area Number |
19K06685
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44020:Developmental biology-related
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| Research Institution | Osama Woman's and Children's Hospital |
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Principal Investigator |
Yoshida Chiharu 地方独立行政法人大阪府立病院機構大阪母子医療センター(研究所), 病因病態部門, 主任研究員 (60360666)
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| Project Period (FY) |
2019-04-01 – 2024-03-31
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| Keywords | 神経管閉鎖不全 / 脱ユビキチン酵素 / マウス / 表皮形成 |
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| Outline of Final Research Achievements |
We have proposed that there are two key processes in epidermal formation during neural tube closure. First, there is the “fate determination of undifferentiated ectodermal cells to epidermal cells,” followed by the “generation of specific epidermal cells. Grhl3 gene, the master factor for epidermal formation, is localized in the nucleus at the time of epidermal cell fate determination and interacts with the canonical Wnt pathway as a transcription factor. Subsequently, GRHL3 factor localizes to the cytoplasm and works with the non-canonical Wnt pathway (PCP pathway) to form specific epidermal cells rich in cytoskeleton. Based on these results, we aim to elucidate what molecules are involved in the migration of GRHL3 factor from the nucleus to the cytoplasm.
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| Free Research Field |
発生生物学
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| Academic Significance and Societal Importance of the Research Achievements |
我々が明らかにした一つの分子が核から細胞質へ局在を変えることで異なるシグナル経路を活性化し、細胞分化と細胞形態変化を制御するシステムは表皮に限らず、他の器官形成や癌、免疫においても同様な機序が働いている可能性がある。このシステムが明らかにできれば非常に新規性が高い知見となる。
一方、神経管閉鎖不全は外科的手術以外に治療法は無く、妊娠時の葉酸服用という予防的な医療が進められているが葉酸の補充では予防し得ない二分脊椎が存在し、現況以上の大幅な発症頻度の減少は期待出来ない。そこで本研究が遂行されることで神経管閉鎖不全症候群の新規な発症予防法・症状の軽減緩和方法に道を開くことを期待している。
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