2021 Fiscal Year Final Research Report
Analysis of spatio-temporal regulation of regeneration and stem cell reprograming factor
| Project/Area Number |
19K06728
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | シロイヌナズナ / 植物ホルモン / 組織癒合 / 接ぎ木 / 時空間的解析 / レーザーマイクロダイセクション |
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| Outline of Final Research Achievements |
After wounding, plant wounded tissues acquire stem cell functions and recognize spatial location information to regenerate the tissues in the appropriate location. However, it is still unclear which cells can become stem cells and the regulatory mechanism of gene expression to regenerate tissues. In this research, We isolated different tissues/specific cells from cryosections of incised Arabidopsis flowering stems by LMD for spatiotemporal quantitative plant hormone analysis, and spatiotemporal quantitative plant hormone analysis and gene expression were performed. In addition, we investigated the molecular and morphological change in the incised flowering stem to reveal the physiological function of ANAC071 and ANAC096 for the regeneration of wounded tissue. We concluded that ANAC071 and ANAC096 are essential for wound-induced cambium formation from dedifferentiated cells before the initiation of cell division during the tissue-reunion process.
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| Free Research Field |
植物生理学
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| Academic Significance and Societal Importance of the Research Achievements |
植物は傷害によって失われた組織を速やかに再生し、分離された組織を癒合させる高い再生力をもっている。この組織修復のメカニズムを応用した技術が接ぎ木であり、栽培期間の短縮、安定した収量・品質の維持や病害抵抗性の付与などを目的とした農業技術として世界的で利用されている。しかし、組織が再生にいたる一連の分子メカニズムについては不明な点が多いほか、農園芸技術への応用についても課題が多い。本研究では、ANAC転写因子と植物ホルモンの関与に注目し、植物の高い再生力のメカニズム解明を目指した。この成果は、接ぎ木技術などの農園芸技術の開発につながることが期待される。
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