2021 Fiscal Year Final Research Report
Cell type-specific histone modification analysis system to elucidate epigenetics leading to dedifferentiation.
| Project/Area Number |
19K06748
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44040:Morphology and anatomical structure-related
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
Sakamoto Takuya 東京理科大学, 理工学部応用生物科学科, 講師 (40637691)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | ヒストン修飾 / mintbody |
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| Outline of Final Research Achievements |
In Arabidopsis, we have successfully developed functional mintbody for the epigenetic mark H3K4me3 and phosphorylation of RNA polymerase IISer2 (RNAPIISer2P), which are indicators of transcriptional activation. Mintbody is a biosensor that detects modification changes using modification-specific intracellular antibodies and fluorescent probes. Both mintbody can quantify the modification level at the cellular level by imaging, and we were able to show that they are useful for monitoring each modification during plant development and other processes. In the future, it is expected that mintbody will be used for detailed epigenetic analysis of each specific cell type.
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| Free Research Field |
植物分子細胞生物学、植物分子生理学
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| Academic Significance and Societal Importance of the Research Achievements |
これまでに、動物細胞や植物の培養細胞において、mintbodyが機能することが報告されてきたが、植物体での報告はなかった。本研究成果により植物体でmintbodyが機能することを初めて示すことができた。また、RNAポリメラーゼIISer2のリン酸化に対するmintbodyは、動植物を通じて初めてゲノムワイドな修飾の解析にも利用できることを示すことができた。今後、mintbodyを特定の細胞種のみで発現させることにより、従来のセルソーティングなどの手法よりも簡便に特定の細胞種毎の詳細なエピジェネティック解析への展開が期待される。
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