Evolution of floral clock genes in Hemerocallis

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K06823
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 45030:Biodiversity and systematics-related
• Research Institution: Azabu University
• Principal Investigator: Nitta Kozue 麻布大学, 生命・環境科学部, 助教 (60589448)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: 開花時間 / 植物ゲノム / ゲノミクス / トランスクリプトーム / 送粉 / 種分化 / 概日時計 / 時計遺伝子

Outline of Final Research Achievements

Hemerocallis fulva are day-blooming flowers that bloom in the early morning and close in the evening. On the other hand, Hemerocallis citrina is a nocturnal flower that begins to bloom in the evening and closes the following morning. In this study, we performed whole-genome sequencing of these species using a Chromium (10x Genomics) sequencer, with the aim of identifying the "floral clock gene" that is involved in the difference in these flowering time. The genome structure was more complex than expected. We compared the sequences of clock genes involved in the circadian clock.

Free Research Field

進化生態学

Academic Significance and Societal Importance of the Research Achievements

本研究で、４Gbp以上の比較的大きなゲノムサイズの解析に挑戦したことは、植物ゲノム研究への貢献も大きいと期待される。ゲノム解析を達成することは、近縁種をはじめ、この属の進化の解明に大きな知見をもたらす。また、１日のなかの開花時間の違いという、植物の生物時計と開花現象の関係を明らかにすることで、植物の花の咲く時間の理解が深まった。