2022 Fiscal Year Final Research Report
Analysis of the evolutionary mechanism of mating systems focusing on the chromosomal structure and sex-determining regions in Closterium.
Project/Area Number |
19K06827
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 45030:Biodiversity and systematics-related
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Research Institution | The University of Tokyo (2020-2022) Chiba University (2019) |
Principal Investigator |
Tsuchikane Yuki 東京大学, 大学院理学系研究科(理学部), 特任研究員 (20434152)
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Keywords | 生殖様式 / ホモタリズム / ヘテロタリズム / ゲノムサイズ / ミカヅキモ / 染色体 |
Outline of Final Research Achievements |
Members of the Closterium peracerosum-strigosum-littorale (C. psl.) complex are unicellular zygnematophycean algae. A zygospore is typically formed as a result of conjugation between mating-type plus (mt+) and mating-type minus (mt-) cells during sexual reproduction in heterothallic strains. On the other hand, zygospores are formed between genetically identical cells in homothallic strains. In this study, We measured the 1C genome size of the C. psl. complex. Genomes of the 6 heterothallic strains and 2 homothallic strains were sequenced. The genome structure of homothallic strain (NIES-4550) indicated that this strain was derived from a hybrid of different heterothallic strains.
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Free Research Field |
進化生物学、藻類学、生理学
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Academic Significance and Societal Importance of the Research Achievements |
ヒメミカヅキモが属する接合藻類は陸上植物に最も近縁な藻類であり、植物の進化を考える上で注目されている。本研究は接合藻類のモデル生物としてヒメミカヅキモに注目し、複数の野生株におけるゲノム量と染色体構造を明らかにした。また、ロングリードシーケンスによる正確なゲノム情報を得ることに成功し、細胞生物学的、分子生物学的基盤が整備された。これらの成果から陸上植物と接合藻類の比較解析も可能となり、今後、接合藻類を用いた進化生物学的な解析の発展が期待される。
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