Construction of efficient systems for generation of anti-metatypic antibody for high-sensitive detection of small molecules

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: Kobe Pharmaceutical University
• Principal Investigator: Oyama Hiroyuki 神戸薬科大学, 薬学部, 准教授 (80572966)
• Co-Investigator(Kenkyū-buntansha): 森田 いずみ 神戸薬科大学, 薬学部, 講師 (20299085) ; 小林 典裕 神戸薬科大学, 薬学部, 教授 (90205477)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 免疫測定法 / 抗メタタイプ抗体 / scFv / 抗体ライブラリー / ハプテン / サンドイッチELISA

Outline of Final Research Achievements

Immunoassay using antigen-antibody reactions are frequently used in clinical testing and diagnostics. Sandwich ELISAs use two different types of antibodies to achieve high sensitivity and specificity, but in principle it has been difficult to apply them to small molecules (haptens). Anti-metatypic antibodies are special antibodies that recognize antigen-antibody complexes and are the key material to establishing sandwich ELISAs for haptens. However, it was difficulted to generation them by the conventional method of immunizing animals. In this study, an array-type screening method was used to prepare an efficient system for generating mutant antibodies that exhibit anti-metatypic activity from antibody-displaying phage libraries. The mutant antibodies obtained enabled sandwich ELISA for thyroxine.

Free Research Field

抗体工学

Academic Significance and Societal Importance of the Research Achievements

研究は抗体ライブラリーの活用と目的の抗体分子種を選択する新たな方法の組み合わせであり、類例はほとんどない。免疫複合体に高い選択性を示す抗メタタイプ抗体の効率的な調製は、従来不可能であったステロイドやビタミン類のサンドイッチ型測定法の構築が容易となる。結果として、検体の測定感度が飛躍的に改善されるうえ操作性にも優れ、臨床診断や現場分析に大きく貢献すると思われる。