Mechanism of Exosome Production by Heterotrimeric G Protein Signaling

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07043
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
• Research Institution: Gifu University
• Principal Investigator: Ueda Hiroshi 岐阜大学, 工学部, 教授 (50253779)
• Co-Investigator(Kenkyū-buntansha): 赤尾 幸博 岐阜大学, 大学院連合創薬医療情報研究科, 特任教授 (00222505)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: GPCR / エクソソーム / PKA

Outline of Final Research Achievements

In recent years, it has been reported that extracellular signaling by exosomes containing RNA and proteins is employed in various cells to induce various cellular responses. It is also known that exosomes are highly relevant to pathological conditions such as cancer, and elucidation of the exosome production mechanism may lead to the treatment of these pathological conditions. In this study, we investigated the mechanism of exosome production by GPCR stimulation and attempted to identify molecules involved in promoting and inhibiting exosome production. We found that there is a regulatory mechanism of exosome production by Gs signaling that is independent of cAMP-dependent protein kinases.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

本研究では、エクソソーム産生機構の一つとして、三量体Gタンパク質共役型受容体(GPCR)刺激の中でも、Gsシグナルに関わる経路について検討した研究であり、過去、そのような研究報告はほとんどない。さらに、エクソソームの放出機構について、促進と抑制に関わる分子の同定を試みている点でも、これらの結果を利用し、今後、がんに関わる治療薬開発や、エクソソームを利用した薬物送達にも応用できると考えられる。