Oxidatively modified high-density lipoprotein: its roles in vessel wall tissues and mechanism of its generation.

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07051
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
• Research Institution: Showa University
• Principal Investigator: ITABE Hiroyuki 昭和大学, 薬学部, 教授 (30203079)
• Co-Investigator(Kenkyū-buntansha): 澤田 直子 (笹部直子) 昭和大学, 薬学部, 助教 (50643566) ; 小濱 孝士 昭和大学, 薬学部, 准教授 (60395647) ; 牧山 智彦 昭和大学, 薬学部, 助教 (60733102)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 酸化LDL / 酸化HDL / 動脈硬化症 / 急性心筋後続 / LC-MS/MS / 好中球細胞外トラップ

Outline of Final Research Achievements

We previously reported the oxidized LDL (oxLDL) isolated from human plasma has a novel structure composed of oxLDL and oxHDL particles. When LDL prelabeled with deuterium-labeled oxidized PC (oxPC) was incubated with HDL, oxPC was quickly hydrolyzed enzymatically and the resulting lysoPC was reacylated by LCAT to form PC. Both oxPC and lysoPC were transferred from LDL to HDL particles spontaneously, and deuterium-labeled PC increased in HDL. NETosis, a novel neutrophil cell death which is related to endothelial damages and thrombosis, was found to be enhanced by the oxLDL-oxHDL complexes isolated from human plasma.

Free Research Field

薬学生物学

Academic Significance and Societal Importance of the Research Achievements

急性心筋梗塞などの重篤な循環器疾患を引き起こす重要なリスク因子であるoxLDLが、生体内においてはどのような性状を有するのかを明らかにすることで、疾患の早期発見や有効な治療法開発に向けた糸口を見出すことが可能になる。