2022 Fiscal Year Final Research Report

Analyses of selenium status and redox control for evaluation of atrophy and insulin resistance with aging

Research Project

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Project/Area Number	19K07073
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
Research Institution	Setsunan University
Principal Investigator	Ueno Hitoshi 摂南大学, 薬学部, 教授 (20176621)
Co-Investigator(Kenkyū-buntansha)	荻野泰史摂南大学, 薬学部, 助教 (80617283) 村野晃一地方独立行政法人大阪健康安全基盤研究所, 衛生化学部, 研究員 (50827277)
Project Period (FY)	2019-04-01 – 2023-03-31
Keywords	セレン / レドックス / 糖尿病 / サルコペニア / フレイル
Outline of Final Research Achievements	The study was performed to identify the selenoproteins that are directly related to an induction of insulin resistance due to aging. Although the NSY mouse model of insulin resistance exhibited the significant decrease in glucose intolerance and the induction of insulin resistance by high fat diet (HFD) ingestion-induced obesity, the senescence-accelerated mice (SAM)P1 did not exhibit such effects. In comparison of SAMP1 with SAMR1 as the control, however, a genetically accelerated aging was responsible to the significant decreases in motility function and selenoprotein (Sel)W expression in gastrocnemius, as well as the induction of insulin resistance. There were also significant increases in serum concertations of SelP by SeMet + HFD administration of NSY mice, whereas the GPx3 was augmented by supplementary SeMet only. The result indicated that the blood secretion of SelP would be rather an indicator of metabolic syndrome.
Free Research Field	公衆衛生学
Academic Significance and Societal Importance of the Research Achievements	セレンは微量必須元素の一つであり、セレンタンパク質は酸化ストレス防御系を担う重要な酵素である。また、セレノプロテインPは肝臓で分泌される血漿中セレンタンパク質であり、従来はセレンの全身運搬・貯蔵として機能すると考えられてきた。日本人はセレン摂取量が比較的多いため、これらのタンパク質は体内で比較的多く発現していると考えられる。本研究において、加齢に伴う筋力低下とインスリン抵抗性との関係が明らかになるとともに、血中SelPが内臓脂肪症候群におけるインスリン抵抗性の指標となることが示唆されたことは学術的意義が大きく身体的フレイル予防のためのバイオマーカー開発の一助になることが期待される。