Elucidation of the mechanism of action and the target molecules of ingredients in crude extracts by chemical genetics approach using yeast knock-out strain collection

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K07145
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47050:Environmental and natural pharmaceutical resources-related
• Research Institution: Nagasaki International University
• Principal Investigator: UTO Takuhiro 長崎国際大学, 薬学部, 准教授 (90469396)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: ケミカルジェネティクス / 出芽酵母 / 生薬エキス / カンゾウ / オウレン / オウゴン / 相互作用

Outline of Final Research Achievements

In this study, we used the chemical genetics approach using approximately 4000 yeast knock-out strain collection to identify the mechanism of action and the target molecules of ingredients in crude extracts, including Glycyrrhiza (Licorice), Coptis rhizome, Scutellaria root. Analyses of their variation patterns demonstrated that these crude drug extracts acted on genes and pathways different from those of representative drugs and chemicals. Furthermore, the functions and subcellular localization of the genes of the sensitive strains were diverse and specific, depending on each crude drug extract.

Free Research Field

生薬学

Academic Significance and Societal Importance of the Research Achievements

本研究は、ケミカルジェネティクス的手法を用いて生薬エキスが約4000の遺伝子群に与える影響を網羅的に解析する初の試みである。本研究により、生薬エキスにより与える遺伝子群は複雑かつ多岐にわたるとともに、一部では共通する部分も見られた。今後、より詳細な検討を要するが、本研究が、生薬・漢方エキスなどの多成分系薬物の作用機序解明の一助になり得ると考える。