Qualitative and quantitative of low-and medium-molecular weight metabolites in exosomal fraction of serum and its application to schizophrenia-risk diagnosis

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07175
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47060:Clinical pharmacy-related
• Research Institution: Toho University
• Principal Investigator: ONOZATO Mayu 東邦大学, 薬学部, 講師 (50610094)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 血清 / エクソソーム / LC-MS/MS / 誘導体化 / チオール化合物 / アミノ酸 / ARMS / 統合失調症

Outline of Final Research Achievements

Decrease in the serum methionine/homocysteine ratio may be a risk factor for developing psychosis during the transition from at-risk mental state (ARMS) to first-episode psychosis (FEP), and may prove to be a useful marker of the phase between ARMS and FEP.
A composition of thiol-containing amino acids in exosomal fraction of human serum was similar to those in the corresponding native serum.
A novel derivatization reagent, CIMa-OSu, is able to determine trace D-amino acids, and furthermore, to distinguish peaks derived from primary amino acids from other amines easily.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

ARMS群のエクソソームに関する報告はほとんどないため、本成果は新たな同疾患の診断法として医療や生命科学研究の発展に繋がる可能性がある。血清エクソソームの定量値の異常が分かれば、統合失調症の発症機構解明に繋がることも期待できる。新規アミノ酸標識化試薬の活用は、エクソソームに含まれるペプチド解析、特に新規ペプチドの同定にも有効であると考えられる。