2021 Fiscal Year Final Research Report
Characterization of immune activation and induction of adaptive immune responses by simian virus 40 virus-like particles
| Project/Area Number |
19K07201
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 47060:Clinical pharmacy-related
|
|---|
| Research Institution | Saitama Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2019-04-01 – 2022-03-31
|
| Keywords | Polyomavirus / Simian virus 40 / VP1 / Virus-like particle / Adjuvant / Adaptive immune response / Antibody production / Cell-mediated immunity |
|---|
| Outline of Final Research Achievements |
Simian virus 40 (SV40) is a monkey polyomavirus. The icosahedral capsid of SV40 is 50 nm in diameter and consists only of major capsid protein SV40 VP1. In this study, we prepared specific antigen incorporated SV40 VLPs (Virus-like particles) from insect cells using baculovirus vector and addressed cell-mediated immunity and antibody production against the incorporated antigens. We revealed that SV40 VLP could induce adaptive immune responses against incorporated antigens without inducing the inflammatory cytokines. In order to reveal the SV40 specific immune responses, we prepared empty SV40 VLP from insect cells and analyzed T-helper differentiation of SV40 VLP-treated naive CD4+ T cells and cytokine/chemokine production by SV40 VLP-treated B cells, CD8+ T cells, natural killer cells, and peritoneal macrophages. In order to reveal the precise molecular mechanism of the SV40 VLP specific immune responses, we also analyzed the cellular proteins that interact with the SV40 VLP.
|
| Free Research Field |
免疫学
|
| Academic Significance and Societal Importance of the Research Achievements |
外来抗原を内包したSimian virus 40 (SV40) のウイルス様粒子 (VLP, virus-like particle) をマウスに免疫すると、外来抗原に対して抗原賦活物質を加えることなくアラムアジュバントと同等の抗体産生を誘導し、また、古典的な不完全フロイントアジュバントによる細胞性免疫誘導よりも効率良く細胞性免疫を誘導することが判明した。また、SV40 VLPに特有の免疫活性化機構として、抗原提示活性化因子の発現上昇とケモカインの産生を誘導することを発見し、SV40 VLPと結合する細胞性因子も同定した。これらの結果は、新規の免疫活性化誘導法の開発に有用であると考えられる。
|
