2021 Fiscal Year Final Research Report
Structural study of Mediator CDK module
| Project/Area Number |
19K07246
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 48010:Anatomy-related
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| Research Institution | Kobe University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 転写 / クライオ電子顕微鏡単粒子 / 超分子複合体 / 難溶性タンパク質 |
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| Outline of Final Research Achievements |
The transcription mediator complex is a multi-protein complex that regulates RNA polymerase II (Pol II) transcription. The transcription mediator complex is composed of four modules with more than 25 subunits, of which the CDK module, composed of four subunits, activates transcription by phosphorylation through the kinase domain and also represses through interaction with other proteins. In this study, we developed a new multi-protein complex preparation technique and analyzed the structure of the CDK module by cryo-EM single-particle analysis to elucidate one aspect of its phosphorylation function.
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| Free Research Field |
構造生物化学
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| Academic Significance and Societal Importance of the Research Achievements |
超分子複合体は転写におけるPol II, 翻訳における Ribosome, スプライシングにおけるスプライソソームのように, 生命活動の重要な役割を担う. 組換えタンパク質発現系を用いた超分子複合体の調製方法は, それらの研究には必須であり, 本研究により開発された方法を適用可能である. CDKモジュールはPol II転写制御の重要因子であり, その分子メカニズムの解明はメディエーター機能の不全を原因とする疾患研究の土台となる.
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