CG-NAP function in ciliogenesis through regulation of centriolar satellite formation

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K07356
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: Teikyo Heisei University
• Principal Investigator: Kim Honsong 帝京平成大学, 薬学部, 講師 (70469899)
• Co-Investigator(Kenkyū-buntansha): 松尾 和彦 京都府立医科大学, 医学(系)研究科(研究院), 助教 (70599753) ; 高橋 美樹子 帝京平成大学, 薬学部, 教授 (90324938)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 一次繊毛 / 中心小体サテライト / AKAP450

Outline of Final Research Achievements

Primary cilia are antenna-like membrane projections that are present in most cells in vivo, one per cell, and serve as cellular sensors, but the process of their formation remains largely unknown. Granular structures called centriolar satellites (CSs) are involved in the transport of factors necessary for the formation of primary cilia. We newly found that CG-NAP, a protein that localizes to the centrosome and Golgi apparatus, is required for the localization of PCM1, the main component of CS, in the vicinity of the Golgi apparatus. CG-NAP knockout cells showed excessive accumulation of CS components near the centrosomes and impaired primary cilia formation, indicating that CG-NAP regulates the proper transport of PCM1 between the Golgi and centrosome, either directly or via microtubules, which is important for the formation of primary cilia.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

一次繊毛の形成に関わる分子の遺伝子変異は、腎嚢胞、肝臓・胆管異常、糖尿病などの症状を呈する繊毛病を引き起こすことが知られている。なかでも一次繊毛の組み立てに関わる分子の細胞質における輸送は、繊毛の形成に必須のプロセスであり、繊毛病の発症に密接に関係するといえる。本研究成果よりCG-NAPとCG-NAP依存的に形成されたゴルジ微小管が繊毛分子の輸送調節に関わることが新たに明らかになった。今後、CG-NAPと微小管が関わる繊毛因子の輸送調節機構の全貌を明らかにしていくことで、繊毛病の発症メカニズムの理解につながることが期待される。