2021 Fiscal Year Final Research Report
Establishment of for evaluation model of pathological subtypes in lung adenocarcinoma using organoid culture and investigation of formation mechanism
Project/Area Number |
19K07414
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 49020:Human pathology-related
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Research Institution | Tottori University |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 浸潤性肺腺癌 / 充実型増殖部 / RNA-Seq / 転写因子 |
Outline of Final Research Achievements |
In this study, we aimed to elucidate the mechanism of pathological subtype formation in invasive lung adenocarcinoma (LUAD). Comparison of gene expression profiles between acinar component and solid component identified 1,272 differentially expressed genes in solid component. Genes involved in cell proliferation were abundant among the upregulated genes, and their expression profiles were consistent with the pathological findings in solid component. Additionally, 10 transcription factors were extracted by in silico analysis that seem to be involved in the regulation of solid component. Among them, four transcription factors significantly increased cell proliferation in LUAD cell lines, suggesting that these transcription factors are involved in the formation and maintenance of solid component in LUAD.
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Free Research Field |
腫瘍生物学
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Academic Significance and Societal Importance of the Research Achievements |
浸潤性肺腺癌において、充実型増殖部を含む一部の病理学的組織亜型の存在は患者予後不良と相関を示すことが報告されているが、その形成機序は未だ不明であり、特異的な治療も存在していない。本研究で明らかにした充実型増殖部の病理所見と一致する遺伝子発現プロファイルは、新たな観点に基づいた肺腺癌治療法開発の基盤になると考えている。さらに、実際に同定した転写因子が肺腺癌の増殖能を増加させたことから、治療標的として有用であると推測でき、今後さらなる研究を重ねることで新規治療法開発へと応用できることが期待できる。
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