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2020 Fiscal Year Research-status Report

Identifying malaria vaccine candidate antigens using genetic linkage analyses

Research Project

Project/Area Number 19K07526
Research InstitutionEhime University

Principal Investigator

カレトン リチャード  愛媛大学, プロテオサイエンスセンター, 教授 (10503782)

Project Period (FY) 2019-04-01 – 2022-03-31
KeywordsPlasmodium vinckei
Outline of Annual Research Achievements

We have generated a comprehensive genetic resource for P. vinckei comprising of five reference-quality genomes, one for each of its subspecies, blood-stage RNA sequencing data for five P. vinckei isolates, and genotypes and growth phenotypes for ten isolates. Additionally, we sequenced seven isolates of the RMP species Plasmodium chabaudi and Plasmodium yoelii, thus extending genotypic information for four additional subspecies enabling a re-evaluation of the genotypic diversity and evolutionary history of RMPs.

The five subspecies of P. vinckei have diverged widely from their common ancestor and have undergone large-scale genome rearrangements. Comparing P. vinckei genotypes reveals region-specific selection pressures particularly on genes involved in mosquito transmission. Using phylogenetic analyses, we show that RMP multigene families have evolved differently across the vinckei and berghei groups of RMPs and that family-specific expansions in P. chabaudi and P. vinckei occurred in the common vinckei group ancestor prior to speciation. The erythrocyte membrane antigen 1 and fam-c families in particular show considerable expansions among the lowland forest-dwelling P. vinckei parasites. The subspecies from the highland forests of Katanga, P. v. vinckei, has a uniquely smaller genome, a reduced multigene family repertoire and is also amenable to transfection making it an ideal parasite for reverse genetics. We also show that P. vinckei parasites are amenable to genetic crosses

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

I have now completed the genomic, phenotypic and transcriptomic characterisation of many new strains of P. vinckei, used them to compile a new and definitive rodent malaria phylogeny, and made these available to the malaria research community. I have published this work in the prestigious BMC Biology journal

Strategy for Future Research Activity

I now intend to characterise further strains of P. vinckei and optimise their transmission to mosquitoes in order to conduct genetic crosses.

Causes of Carryover

Next year's budget will be spent on publication fees, plastic consumables, insectary maintenance, molecular biological reagents, and mice.

  • Research Products

    (3 results)

All 2021 2020

All Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results)

  • [Journal Article] Plasmodium vinckei genomes provide insights into the pan-genome and evolution of rodent malaria parasites2021

    • Author(s)
      Ramaprasad Abhinay、Klaus Severina、Douvropoulou Olga、Culleton Richard、Pain Arnab
    • Journal Title

      BMC Biology

      Volume: 19 Pages: -

    • DOI

      10.1186/s12915-021-00995-5

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Malaria parasites regulate intra-erythrocytic development duration via serpentine receptor 10 to coordinate with host rhythms2020

    • Author(s)
      Subudhi Amit K.、O’Donnell Aidan J.、Ramaprasad Abhinay、Abkallo Hussein M.、Kaushik Abhinav、Ansari Hifzur R.、Abdel-Haleem Alyaa M.、Ben Rached Fathia、Kaneko Osamu、Culleton Richard、Reece Sarah E.、Pain Arnab
    • Journal Title

      Nature Communications

      Volume: 11 Pages: -

    • DOI

      10.1038/s41467-020-16593-y

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Consequences of Mixed-Species Malaria Parasite Co-Infections in Mice and Mosquitoes for Disease Severity, Parasite Fitness, and Transmission Success2020

    • Author(s)
      Tang Jianxia、Templeton Thomas J.、Cao Jun、Culleton Richard
    • Journal Title

      Frontiers in Immunology

      Volume: 10 Pages: -

    • DOI

      10.3389/fimmu.2019.03072

    • Peer Reviewed / Open Access / Int'l Joint Research

URL: 

Published: 2021-12-27  

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