2021 Fiscal Year Final Research Report
Functional analysis of Beclin 1 regulating different stages during bacterial infection
| Project/Area Number |
19K07537
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
野澤 孝志 京都大学, 医学研究科, 准教授 (10598858)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | A群レンサ球菌 / Beclin 1 / 細胞侵入 |
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| Outline of Final Research Achievements |
Beclin 1 is important for the induction of both autophagy, an intracellular non-selective autophagosomal degradation mechanism induced during nutrient starvation, and selective autophagy induced during bacterial infection. We have previously shown that Beclin 1 regulates bacterial host cell invasion, and in this study, we identified ECD as the domain responsible for regulating cell invasion by Group A Streptococcus (GAS). In addition, detailed molecular analysis in GAS-infected cells revealed that GAS activates ILK1 through binding to host cell integrins, which in turn regulates GAS cell invasion via activation of AKT and finally Beclin 1-ECD.
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| Free Research Field |
細菌学
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| Academic Significance and Societal Importance of the Research Achievements |
Beclin 1が細菌に対する選択的オートファジーの制御だけでなく、細菌の宿主細胞への侵入制御にも関与することは、本分子が異なる感染ステーシにおいて重要なマルチロールプレーヤーとして宿主の感染制御に貢献していることを示唆している。本研究で得られた結果は、細菌と宿主間の相互作用を明らかにする上で学術的に意義のあるものであり、また細菌感染を制御する新たな薬剤の標的としてBeclin 1およびその相互作用分子の有用性を示したことは、社会的に意義があったと考えられる。
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