2021 Fiscal Year Final Research Report
Comprehensive analysis of the mechanism of persistent infection of Leptospira interrogans in rats using transposon insertion mutants
| Project/Area Number |
19K07571
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Koizumi Nobuo 国立感染症研究所, 細菌第一部, 主任研究官 (10333361)
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| Co-Investigator(Kenkyū-buntansha) |
中村 修一 東北大学, 工学研究科, 准教授 (90580308)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | レプトスピラ / 持続感染 |
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| Outline of Final Research Achievements |
Tn-seq using transposon mutant library of Leptospira interrogans serovar Manilae identified 129 mutants whose capacity to colonize kidney tissues of rats were reduced. When mutant strain RS00325 or RS01180 were inoculated with their parental strain to rats, the number of RS00325 and RS01180 mutants in blood at three days post infection was reduced to 1/1000 and 1/60 compared to the parental strain.
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| Free Research Field |
細菌学
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| Academic Significance and Societal Importance of the Research Achievements |
レプトスピラの宿主持続感染機構を解明することは，本菌が引き起こす疾患であるレプトスピラ症のコントロールのために重要である．本研究で得られた変異体の解析により持続感染に関わるレプトスピラ因子を明らかにすることで，それらをターゲットとしたワクチンや治療薬の開発も期待される．
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