2019 Fiscal Year Research-status Report
Viral regulation of host RNA degradation and its implication on hepatocarcinogenesis.
| Project/Area Number |
19K07586
|
|---|
| Research Institution | National Institute of Infectious Diseases |
|---|
Principal Investigator |
アリ フセインハッサン 国立感染症研究所, ウイルス第二部, 主任研究官 (00523515)
|
|---|
| Project Period (FY) |
2019-04-01 – 2022-03-31
|
| Keywords | HBx / Ski2 / RNA degradation / HBV / Viral life cycle |
|---|
| Outline of Annual Research Achievements |
By controlling mRNA stability, the virus can manipulate cellular environment to favor its persistence.Ski2 is an essential helicase for cytoplasmic RNA degradation through the RNA exosome system. HBx regulated Ski2 expression.These data suggested a possible effect of HBx on the mRNA stability,and the resulting regulation of gene expression, for both viral and host RNA that is degraded through the RNA exosome system.In 2019, we analyzed the role of HBx on the regulation of other HBV proteins expression through the regulation of Ski2-mediated mRNA decay (using expressing vectors for HBV, HBV lacking the expression of HBx, and siRNA targeting Ski2). Functional analysis is undergoing to analyze its regulation on the viral life cycle.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We clarified the role of HBx on the expression of other HBV proteins through the regulation of Ski2-mediated RNA degradation in 2019. All tools required to perform the study were available, and the financial support was enough to reach this target.
|
| Strategy for Future Research Activity |
In 2020, we aim to perform two major lines of experiments. On the viral side, we wish to clarify the functional significance of HBx-mediated regulation of the expression of other HBV proteins through its regulation of RNA-Exosome mediated RNA degradation. While on the host side, we will perform deep sequencing to identify the host RNA transcriptome that is affected by HBx regulation of RNA-exosome mediated RNA degradation; confirm RNA-sequencing results by quantitative real time PCR, and start to analyze possible role of these host genes on viral life cycle, and/or viral-induced carcinogenesis.
|
-
-
-
[Presentation] MafF is a key player of IL-1β-induced suppression of transcription from HBV-core promoter, and the resulting suppression of viral replication2019
Author(s)
2.Marwa K. Ibrahim, Sameh A. Gad, Koichi Watashi, Li Yingfang, Masaya Sugiyama, Masahiko Ito, Asako Murayama, Tetsuro Suzuki, Takanobu Kato, Kunitada Shimotohno, Masamichi Muramatsu, Takaji Wakita, Hussein H. Aly.
Organizer
2019 International Meeting, molecular Biology of Hepatitis B virus
Int'l Joint Research
-
[Presentation] The IL-1 β induced (MafF) is an important regulator of HBV core promoter activity.2019
Author(s)
1.Marwa K. Ibrahim, Sameh A. Gad, Koichi Watashi, Li Yingfang, Masaya Sugiyama, Masahiko Ito, Asako Murayama, Tetsuro Suzuki, Takanobu Kato, Kunitada Shimotohno, Masamichi Muramatsu, Takaji Wakita, Hussein H. Aly
Organizer
The 67th Annual Meeting of the Japanese Society for Virology.
-
[Presentation] MAFF IS AN IMPORTANT REGULATOR OF HBV CORE PROMOTER ACTIVITY, AND HBV REPLICATION2019
Author(s)
Marwa K. Ibrahim, Sameh A. Gad, Koichi Watashi, Takanobu Kato, Asako Murayama, Kunitada Shimotohno, Takaji Wakita, Masamichi Muramatsu, Hussein H. Aly
Organizer
The Liver Meeting 2019, The 70th Annual Meeting of the American Association for the Study of Liver Diseases (AASLD)
Int'l Joint Research
