2022 Fiscal Year Final Research Report
Phosphoproteomic analysis of IFN production mechanism by plasmacytoid dendritic cells
| Project/Area Number |
19K07605
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49070:Immunology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 形質細胞様樹状細胞 / 定量的リン酸化プロテオーム解析 / インターフェロン・アルファ / チロシンキナーゼ阻害剤 / トル様受容体9 |
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| Outline of Final Research Achievements |
Plasmacytoid dendritic cells (pDCs) are characterized by their ability to rapidly produce large amounts of IFN-α and play an important role in biological defense and the pathogenesis of autoimmune and inflammatory diseases. In this study, to elucidate the molecular mechanisms underlying IFN-α production by pDCs, we used our originally established pDC cell lines and performed quantitative phosphoproteomic analysis under two inhibitors, which are either inhibitory or non-inhibitory for IFN-α production, thereby efficiently identifying phosphorylated proteins involved in IFN-α production in pDC.
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| Free Research Field |
免疫学
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| Academic Significance and Societal Importance of the Research Achievements |
形質細胞様樹状細胞(pDC)は大量のIFN-αを迅速に産生し免疫反応の起点となる細胞で、生体防御や自己免疫疾患・炎症性疾患の病態形成に重要な役割を担っている。この研究ではこれまで困難であったpDCのプロテオーム解析の手法を確立することによって、pDCのIFN-α産生に関与する未知の蛋白を同定した。今後、pDCを特異的に制御する治療を開発することによってワクチンや免疫疾患治療の開発に応用されることが期待される。
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