2021 Fiscal Year Final Research Report
Elucidation of ectopic formation of super enhancers by SATB1 for breast cancer malignancy
| Project/Area Number |
19K07664
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 50010:Tumor biology-related
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| Research Institution | Kyushu University (2020-2021)
Shiga University of Medical Science (2019) |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | スーパーエンハンサー / 乳がん / SATB1 |
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| Outline of Final Research Achievements |
We aimed to determine the association of SATB1 with super-enhancers (SEs) formation around metastasis-related genes during the malignant transformation of breast cancer cells. Forced expression of SATB1 in non-invasive breast cancer cell line MCF-7 transiently indicated malignant phenotypes. It was difficult to use as the cancer progression model. Therefore, we used a lung cancer cell line A549, which can be stably induced epithelial-mesenchymal transition (EMT), to analyze time-dependent SE formation by BRD4 ChIP-Seq. The results indicated that SEs were reorganized during EMT. It was also shown that the motifs of transcription factors enriched in SEs were altered during EMT.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、がん細胞が転移能を獲得する過程で（EMT）、時間経過に伴いSEのゲノムワイドな分配パターンが変化することを示した。SEにエンリッチする転写因子のモチーフはEMTの時間経過に伴い変化することから、特定の転写因子の機能を阻害することでがん細胞の転移能獲得を遅延または阻害できる可能性が示された。マウスやIn vitro実験による検証は必要ではあるが、将来的ながんの治療標的となる可能性が示された。
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