significance of micro RNA as biomarker in primary aldosteronism

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07885
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 52010:General internal medicine-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Yoshimoto Takanobu 東京医科歯科大学, 医学部, 非常勤講師 (80297457)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: アルドステロン / 副腎腫瘍 / マイクロRNA

Outline of Final Research Achievements

The present study was undertaken to comprehensively explore micro(mi)RNAs which are specifically expressed in aldosterone-producing adenoma (APAs), an adrenocortical tumor which causes hypertension through aldosterone hypersecretion. We characterized several unique miRNAs in surgical specimen of APAs by the combination of NGS and qPCR. Among these, we found that miR299 is associated with the regulation of HPSE expression, an enzyme involved in the degradation of heparan sulfate proteoglycan. MicroRNAome stud would be one of the important research methods for elucidating the pathogenesis of APAs.

Free Research Field

内分泌代謝領域

Academic Significance and Societal Importance of the Research Achievements

アルドステロン産生腺腫（APA）の発症機序はいまだ不明な点が多いが、今回のAPA腫瘍組織におけるmiRNAの網羅的発現解析により、複数のmiRNAが特徴的な発現を示している事が明らかとなった。加えていくつかのmiRNAはAPAの病因あるいは病態と関連する各種遺伝子発現と相関しており、特にmiR299については細胞間基質の発現制御を介して腫瘍発生に関与する可能性が新たに示された。miRNAome研究は、APAの病態発生で未解明であった領域のさならなる解明に重要な役割を担うと考えられる。