2022 Fiscal Year Final Research Report
Analysis of intestinal mucosa-associated microbiota involved in progression to non-alcoholic steatohepatitis
| Project/Area Number |
19K07938
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52010:General internal medicine-related
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| Research Institution | Kobe University |
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Principal Investigator |
Sakai Arata 神戸大学, 医学部附属病院, 特定助教 (50792636)
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| Co-Investigator(Kenkyū-buntansha) |
井上 潤 神戸大学, 医学部附属病院, 助教 (50631561)
矢野 嘉彦 神戸大学, 医学研究科, 講師 (60419489)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 非アルコール性脂肪肝 / 腸内細菌 |
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| Outline of Final Research Achievements |
Non-alcoholic fatty liver disease/hepatitis (NAFLD/NASH) is a liver injury with a non-alcoholic fatty liver background. It is known that the gastrointestinal flora is involved in the pathogenesis, but the pathogenesis is unknown. The purpose of this study is to analyze not only the feces of patients but also the bacterial flora on the surface of the intestinal mucosal epithelium, and to clarify the relationship between disease characteristics and disease activity. In this study, bacterial DNA was extracted from NAFLD and healthy subjects' stool, mucus on the surface of the gastrointestinal mucosa, and saliva, and analyzed using a next-generation sequencer. In NAFLD patients, we clarified not only the changes in the fecal flora, as previously reported, but also the characteristics of the mucosal surface bacteria on the enterobacterial side. We believe that the findings obtained in this study can provide basic information for elucidating the pathology of NASH and NAFLD.
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| Free Research Field |
消化器内科学
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| Academic Significance and Societal Importance of the Research Achievements |
近年、非アルコール性脂肪性肝/肝炎（NAFLD/NASH）を背景とした肝硬変や肝癌は増加傾向となっており、社会的な問題となっている。NAFLD/NASHの病態には腸内細菌が関わっていることがこれまで示唆されているが、糞便中の腸内細菌叢を解析した研究に限られており、その全貌は明らかでない。本研究では、消化管粘膜表層の細菌叢の解析を行い、これまでに明らかにされていなかったNAFLD患者での消化管の表層細菌の変化を解析し、NASH・NAFLDの病態解明の基礎的知見が得られた。NASH・NAFLDの病態解明への進展が期待できる成果であると考える。
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