2022 Fiscal Year Final Research Report
Analysis of Alzheimer's disease model neurons focusing on synaptic dysfunction
| Project/Area Number |
19K08010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 52030:Psychiatry-related
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 樹状突起スパイン / ドレブリン / ハイスループット解析 |
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| Outline of Final Research Achievements |
Alzheimer’s disease causes cognitive dysfunction such as dementia, and it is reported that the degree of cognitive dysfunction correlates with synaptic dysfunction. It is important to reveal the synaptic dysfunction in Alzheimer’s disease focusing on dendritic spines, because they are the main structures of excitatory synapses. To study Alzheimer’s disease focusing on dendritic spines, a new animal model of Alzheimer’s disease was made in this study. In addition, as an in vitro analysis system is necessary to study the dendritic spine level of the disease, in vitro high-content imaging analysis system was established. Drebrin, an actin-binding protein that accumulates at dendritic spines, is a good marker for synaptic states. The dendritic spines of cultured neurons from a new animal model of Alzheimer’s disease were analyzed focusing on drebrin. It is suggested that the exodus of drebrin from dendritic spines causes synaptic dysfunction.
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| Free Research Field |
神経科学
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| Academic Significance and Societal Importance of the Research Achievements |
アルツハイマー病脳のシナプス機能不全の実態はその検証があまり進んでいない。本研究ではモデル神経細胞を用いてin vitro解析系を開発し、スパインレベルでの解析を可能とした。今後、さらに検証を進めることでアルツハイマー病におけるシナプス機能不全の実態を明らかにすることができると期待される。さらに本研究では、ドレブリンをシナプス機能マーカーとし、医薬品のシナプス機能安全性評価などへ応用可能なシナプス機能評価のハイスループット化を実現させた。
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