64Cu-ATSM Predicts Efficacy of Carbon Ion Radiotherapy Associated with Cellular Antioxidant Capacity

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K08091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 52040:Radiological sciences-related
• Research Institution: Gunma University
• Principal Investigator: Ohno Tatsuya 群馬大学, 大学院医学系研究科, 教授 (10344061)
• Co-Investigator(Kenkyū-buntansha): 尾池 貴洋 群馬大学, 医学部附属病院, 講師 (10643471) ; 花岡 宏史 関西医科大学, 医学部, 教授 (50361390)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 炭素イオン線 / 64Cu-ATSM / 抗酸化能 / 悪性腫瘍

Outline of Final Research Achievements

We found that the carbon ion RBE correlated with 64Cu-ATSM uptake both in vitro and in vivo. High RBE/64Cu-ATSM cells showed greater steady-state levels of antioxidant proteins and increased capacity to scavenge reactive oxygen species in response to X-rays than low RBE/64Cu-ATSM counterparts; this upregulation of antioxidant systems was associated with downregulation of TCA
cycle intermediates. Furthermore, inhibition of nuclear factor erythroid 2-related factor 2 (Nrf2) sensitized high RBE/64Cu-ATSM cells to X-rays, thereby reducing RBE values to levels comparable to those in low RBE/64Cu-ATSM cells.

Free Research Field

放射線腫瘍学

Academic Significance and Societal Importance of the Research Achievements

がん細胞の抗酸化能は炭素イオン線RBEに寄与すること、ならびに64Cu-ATSM取り込みは同機序を反映した高RBE腫瘍の診断バイオマーカー候補であることが示唆された。64Cu-ATSMイメージングを用いた光子線ならびに高LET放射線治療計画の個別化、高度化が期待される。